AtT-20

 
Species source: mouse
Organ source: pituitary tumor
Plasmid:
  • pcDNA3
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 10% CO2
Antibiotics:
  • Geneticin (G418) - 1000μg/ml - 24h
Reference:
  • S. E. Wolfe, D. E. Howard, J. A. Schetz, C. J. Cheng, R. Webber, D. M. Beatty, B. M. Chronwall and S. J. Morris 1999. Dopamine D2 Receptor Isoforms Expressed in AtT20 Cells Inhibit Q Type High Voltage Activated Ca2+ Channels via a Membrane Delimited Pathway