CD40-B

 
Plasmid:
  • pcDNA3.1
Culture/Media: Iscove's modified Dulbecco's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Puromycin - 0.6μg/ml - 2days
Reference:
  • E. Kondo, M. S. Topp, H. P. Kiem, Y. Obata, Y. Morishima, K. Kuzushima, M. Tanimoto, M. Harada, T. Takahashi and Y. Akatsuka 2002. Efficient generation of antigen-specific cytotoxic T cells using retrovirally transduced CD40-activated B cells