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Cell-Lines
Plasmid/Vector
Antibiotics
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NEC
NEC
Plasmid:
pMAMNeo
pMAMNeo
Culture/Media:
Dulbecco's modified Eagle's medium
Temperature:
37
Atmosphere:
5% CO2
Antibiotics:
Geneticin (G418) - 200μg/ml - 48h
Reference:
W. Yueying, W. Jianbo, L. Hailin, T. Huaijing, G. Liping and L. Shih-Hsin 2008. ECRG1, a novel esophageal gene, cloned and identified from human esophagus and its inhibition effect on tumors