MEFs

 
Plasmid:
  • pcDNA3.1/zeo
Culture/Media: Dulbecco's modified Eagle's medium
Antibiotics:
  • Zeocin - 300μg/ml -
Reference:
  • S. Kondo, Y. Lu, M. Debbas, A. W. Lin, I. Sarosi, A. Itie, A. Wakeham, J. A. Tuan, C. Saris and G. Elliott 2003. Characterization of cells and gene-targeted mice deficient for the p53-binding kinase homeodomain-interacting protein kinase 1 (HIPK1)