MEFs

Cell-Lines
Plasmid/Vector
Antibiotics

Plasmid:	PIG/miR-20a
Culture/Media:	Dulbecco's modified Eagle's medium
Antibiotics:	Puromycin - 2μg/ml - 2days
Reference:	L. Poliseno, L. Pitto, M. Simili, L. Mariani, L. Riccardi, A. Ciucci, M. Rizzo, M. Evangelista, A. Mercatanti and P. P. Pandolfi 2008. The proto-oncogene LRF is under post-transcriptional control of MiR-20a: implications for senescence

This project is supported byTOKU-Ewhich specializes in manufacturing ultra-pure antibiotics for a broad spectrum of biotechnology applications as well as for the pharmaceutical industry. TOKU-E is a global leader in biotechnology innovation, offering great benefits and applications to the biopharmaceutical and diagnostic industries as well as for biotechnology research communities.
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