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Plasmid/Vector
Antibiotics
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Plasmid:
pIRES-NaPi-IIa-FLAG
Culture/Media:
Dulbecco's modified Eagle's medium
Temperature:
37
Atmosphere:
5% CO2
Antibiotics:
Geneticin (G418) - 2000μg/ml - 10days
Reference:
K. NASHIKI, Y. TAKETANI, T. TAKEICHI, N. SAWADA, H. YAMAMOTO, M. ICHIKAWA, H. ARAI, K. E. N. I. MIYAMOTO and E. TAKEDA 2005. Role of membrane microdomains in PTH-mediated down-regulation of NaPi-IIa in opossum kidney cells