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Plasmid/Vector
Antibiotics
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NPA
NPA
Plasmid:
pSVEts-2
pSVEts/lacZ
pSVlacZ
pSVlacZ
Culture/Media:
Ham’s F-12 nutrient mixture
Antibiotics:
Geneticin (G418) - 400μg/ml - 15days
Reference:
F. de Nigris, T. Mega, N. Berger, M. V. Barone, M. Santoro, G. Viglietto, P. Verde and A. Fusco 2001. Induction of ETS-1 and ETS-2 transcription factors is required for thyroid cell transformation