NPA

 
Plasmid:
  • pSVEts-2
  • pSVEts/lacZ
  • pSVlacZ
  • pSVlacZ
Culture/Media: Ham’s F-12 nutrient mixture
Antibiotics:
  • Geneticin (G418) - 400μg/ml - 15days
Reference:
  • F. de Nigris, T. Mega, N. Berger, M. V. Barone, M. Santoro, G. Viglietto, P. Verde and A. Fusco 2001. Induction of ETS-1 and ETS-2 transcription factors is required for thyroid cell transformation