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Plasmid/Vector
Antibiotics
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ES cell
ES cell
Plasmid:
pTP6
Antibiotics:
Puromycin - 3μg/ml - 14days
Reference:
C. Annerén, C. A. Cowan and D. A. Melton 2004. The Src family of tyrosine kinases is important for embryonic stem cell self-renewal