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Plasmid/Vector
Antibiotics
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K562
K562
Species source:
human
Organ source:
lymphoblastoid
Plasmid:
pUC19
pNL4-3/pBABE.CCR5
Culture/Media:
Dulbecco's modified Eagle's medium
Antibiotics:
Puromycin - 10μg/ml - 6weeks
Reference:
S. Koesters, W. J. Rutherford and K. R. Fowke 2002. Development of a novel allo-independent HIV-1 virus preparation for use in immunoassays