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Cell-Lines
Plasmid/Vector
Antibiotics
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MEFs
MEFs
Plasmid:
pQCXIP
pVSVG
Culture/Media:
Dulbecco's modified Eagle's medium
Antibiotics:
Puromycin - 2μg/ml - 2weeks
Reference:
H. Huang, S. Y. Choi and M. A. Frohman 2010. A quantitative assay for mitochondrial fusion using Renilla luciferase complementation