293

 
Species source: human
Organ source: kidney; fetal
Plasmid:
  • pRc/CMV
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2 and 95% air
Antibiotics:
  • Geneticin (G418) - 400μg/ml - 21days
Reference:
  • S. P. Little, E. P. Dixon, F. Norris, W. Buckley, G. W. Becker, M. Johnson, J. R. Dobbins, T. Wyrick, J. R. Miller and W. MacKellar 1997. Zyme, a novel and potentially amyloidogenic enzyme cDNA isolated from Alzheimer’s disease brain