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Plasmid/Vector
Antibiotics
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HeLa S3
HeLa S3
Species source:
human
Organ source:
cervix; adenocarcinoma
Plasmid:
pcDNA (Hupf1-2tag)
Culture/Media:
Dulbecco's modified Eagle's medium
Antibiotics:
Geneticin (G418) - 600μg/ml - 2days
Reference:
T. Schell, T. Köcher, M. Wilm, B. Seraphin, A. E. Kulozik and M. W. Hentze 2003. Complexes between the nonsense-mediated mRNA decay pathway factor human upf1 (up-frameshift protein 1) and essential nonsense-mediated mRNA decay factors in HeLa cells