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BD EcoPack2-293
BD EcoPack2-293
Plasmid:
pBabe-puro-C/EBP-α
pQCXIP-mTOR
pQCXIP-RR-S6K1
Culture/Media:
Dulbecco's modified Eagle's medium
Antibiotics:
Puromycin - 200μg/ml - 7d
Reference:
J. E. Kim and J. Chen 2004. Regulation of Peroxisome Proliferator–Activated Receptor- Activity by Mammalian Target of Rapamycin and Amino Acids in Adipogenesis