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Antibiotics
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NIH/3T3
NIH/3T3
Species source:
mouse
Organ source:
embryo
Plasmid:
WZLhygro
WZLhygroPPARγ2
Culture/Media:
Dulbecco's modified Eagle's medium
Antibiotics:
Hygromycin B - 400μg/ml - 7d
Reference:
G. Adelmant, J. D. Gilbert and S. O. Freytag 1998. Human translocation liposarcoma-CCAAT/enhancer binding protein (C/EBP) homologous protein (TLS-CHOP) oncoprotein prevents adipocyte differentiation by directly interfering with C/EBP function