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Plasmid/Vector
Antibiotics
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3T3-L1
3T3-L1
Species source:
mouse
Organ source:
embryo
Plasmid:
pL-IRAPHA
Culture/Media:
Dulbecco's modified Eagle's medium
Antibiotics:
Geneticin (G418) - 500μg/ml - 7d
Reference:
D. C. Berwick, G. C. Dell, G. I. Welsh, K. J. Heesom, I. Hers, L. M. Fletcher, F. T. Cooke and J. M. Tavaré 2004. Protein kinase B phosphorylation of PIKfyve regulates the trafficking of GLUT4 vesicles