293T

 
Species source: human
Organ source: Kidney (embryonic)
Plasmid:
  • pRevTRE-C/EBPα-Myc-His
  • pBabe-Puro-LAP
Culture/Media: Dulbecco's modified Eagle's medium
Antibiotics:
  • Puromycin - 2μg/ml - 14-21d
Reference:
  • Y. Zuo, L. Qiang and S. R. Farmer 2006. Activation of CCAAT/enhancer-binding protein (C/EBP) expression by C/EBP during adipogenesis requires a peroxisome proliferator-activated receptor- -associated repression of HDAC1 at the C/ebp gene promoter