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Antibiotics
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MCF-12A
MCF-12A
Species source:
human
Organ source:
mammary gland
Plasmid:
pSilencer 3.1-H1
Culture/Media:
Dulbecco's modified Eagle's medium
Antibiotics:
Puromycin - 0.5μg/ml - 6days
Reference:
D. R. Wonsey and M. T. Follettie 2005. Loss of the forkhead transcription factor FoxM1 causes centrosome amplification and mitotic catastrophe