GP+E-86

 
Species source: mouse
Organ source: embryo; amphotropic retroviral packaging line
Plasmid:
  • pST2
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Geneticin (G418) - 500μg/ml - 10days
Reference:
  • L. Kucerova, M. Matuskova, A. Pastorakova, S. Tyciakova, J. Jakubikova, R. Bohovic, V. Altanerova and C. Altaner 2008. Cytosine deaminase expressing human mesenchymal stem cells mediated tumour regression in melanoma bearing mice