C2C12

 
Species source: mouse
Organ source: muscle
Plasmid:
  • pCR2.1-TOPO
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Geneticin (G418) - 400μg/ml - 2weeks
Reference:
  • K. Yuasa, T. Masuda, C. Yoshikawa, M. Nagahama, Y. Matsuda and A. Tsuji 2009. Subtilisin-like proprotein convertase PACE4 is required for skeletal muscle differentiation