NIH/3T3

 
Species source: mouse
Organ source: embryo
Plasmid:
  • pFLAG-CMV22
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 10% CO2
Antibiotics:
  • Geneticin (G418) - 600μg/ml - 10days
Reference:
  • C. W. Chien and I. Ho 2009. Induction of neoplastic transformation by ectopic expression of human aldo-keto reductase 1C isoforms in NIH3T3 cells