C2C12

 
Species source: mouse
Organ source: muscle
Plasmid:
  • pTet-On
  • pTK-Hyg
  • pTRE
  • pTRE
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Geneticin (G418) - 300μg/ml - 2weeks
  • Hygromycin - 300μg/ml - 2weeks
Reference:
  • Y. Arao, K. Carpenter, S. Hewitt and K. S. Korach 2010. Estrogen Down-regulation of the Scx Gene Is Mediated by the Opposing Strand-overlapping Gene Bop1