PA317

 
Species source: mouse
Organ source: embryo; amphotropic retroviral packaging line
Plasmid:
  • pDONL
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Geneticin (G418) - 400μg/ml - 10-14d
Reference:
  • V. A. Zakhartchenko, W. F. Anderson and Y. Rozenberg 2001. Research Report Quantitation of Retroviral-Mediated Transfer Using Luciferase in Living and Lysed Cells