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Plasmid/Vector
Antibiotics
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3T3-L1
3T3-L1
Species source:
mouse
Organ source:
embryo
Plasmid:
pBMN
Culture/Media:
Dulbecco's modified Eagle's medium
Antibiotics:
Puromycin - 2μg/ml - 5days
Reference:
D. Ren, T. N. Collingwood, E. J. Rebar, A. P. Wolffe and H. S. Camp 2002. PPAR knockdown by engineered transcription factors: exogenous PPAR 2 but not PPAR 1 reactivates adipogenesis