STO

 
Species source: mouse
Organ source: embryo
Plasmid:
  • pIRES2-DsRed
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Neomycin - 200μg/ml - 24h
  • Geneticin (G418) - 200μg/ml - 24h
Reference:
  • H. T. Lin, C. L. Kao, K. H. Lee, Y. L. Chang, S. H. Chiou, F. T. Tsai, T. H. Tsai, D. C. Sheu, L. L. T. Ho and H. H. Ku 2007. Enhancement of insulin-producing cell differentiation from embryonic stem cells using pax4-nucleofection method