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Plasmid/Vector
Antibiotics
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ES cell
ES cell
Plasmid:
CMV-EGFP
Rex1-EGFP
Culture/Media:
Dulbecco's modified Eagle's medium
Antibiotics:
Geneticin (G418) - 200μg/ml -
Reference:
R. Eiges, M. Schuldiner, M. Drukker, O. Yanuka, J. Itskovitz-Eldor and N. Benvenisty 2001. Establishment of human embryonic stem cell-transfected clones carrying a marker for undifferentiated cells