CHO-K1

 
Species source: hamster, Chinese
Organ source: ovary
Plasmid:
  • pCEP4
  • pUMS(ELAM)-Tac
Culture/Media: Ham’s F-12 nutrient mixture
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Hygromycin B - 400units/ml -
Reference:
  • R. L. Delude, A. Yoshimura, R. R. Ingalls and D. T. Golenbock 1998. Construction of a lipopolysaccharide reporter cell line and its use in identifying mutants defective in endotoxin, but not TNF- , signal transduction