293

 
Species source: human
Organ source: kidney; fetal
Plasmid:
  • pcDNA3
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Hygromycin B - 200μg/ml - 21-28days
Reference:
  • E. R. Leight and B. Sugden 2001. Establishment of an oriP replicon is dependent upon an infrequent, epigenetic event