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Cell-Lines
Plasmid/Vector
Antibiotics
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hESCs
hESCs
Plasmid:
pTP6
pTP6-Lefty2
pTP6-Cerb-S
Culture/Media:
KO-DMEM
Antibiotics:
Hygromycin - 1μg/ml - 3days
Reference:
J. R. Smith, L. Vallier, G. Lupo, M. Alexander, W. A. Harris and R. A. Pedersen 2008. Inhibition of Activin/Nodal signaling promotes specification of human embryonic stem cells into neuroectoderm