CHO-K1

 
Species source: hamster, Chinese
Organ source: ovary
Plasmid:
  • pVSVG-T7
Culture/Media: DMEM + F12 medium
Temperature: 37
Atmosphere: 8-9% CO2
Antibiotics:
  • Geneticin (G418) - 700μg/ml -
Reference:
  • A. Nohturfft, D. Yabe, J. L. Goldstein, M. S. Brown and P. J. Espenshade 2000. Regulated step in cholesterol feedback localized to budding of SCAP from ER membranes