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Antibiotics
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NIH3T3
NIH3T3
Species source:
mouse
Organ source:
embryo
Plasmid:
pMSCV
pMSCV/HAPPARγWT
pMSCV/HA-PPARγK422Q
Culture/Media:
Dulbecco's modified Eagle's medium
Antibiotics:
Puromycin - 2μg/ml - 48h
Reference:
R. A. Gupta, P. Sarraf, E. Mueller, J. A. Brockman, J. J. Prusakiewicz, C. Eng, T. M. Willson and R. N. DuBois 2003. Peroxisome Proliferator-activated Receptor -mediated Differentiation