MCF 10A

 
Species source: human
Organ source: mammary gland; fibrocystic disease
Plasmid:
  • LPCX
Culture/Media: DMEM / F 12 medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Puromycin - 2μg/ml - 5days
Reference:
  • F. Tian, S. DaCosta Byfield, W. T. Parks, S. Yoo, A. Felici, B. Tang, E. Piek, L. M. Wakefield and A. B. Roberts 2003. Reduction in Smad2/3 signaling enhances tumorigenesis but suppresses metastasis of breast cancer cell lines