NIH/3T3

 
Species source: mouse
Organ source: embryo
Plasmid:
  • pSV2neo
  • pSGns3/1–180
  • pSGns3/1–180/L106A
  • pSG5
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5%CO2
Antibiotics:
  • Geneticin (G418) - 600μg/ml -
Reference:
  • M. Tanaka, M. Nagano-Fujii, L. Deng, S. Ishido, K. Sada and H. Hotta 2006. Single-point mutations of hepatitis C virus NS3 that impair p53 interaction and anti-apoptotic activity of NS3