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Plasmid/Vector
Antibiotics
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CHO
CHO
Species source:
hamster, Chinese
Organ source:
ovary
Plasmid:
recombinase expression plamid
recombinant assay plasmid
Culture/Media:
Ham’s F-12 nutrient mixture
Temperature:
37
Antibiotics:
Geneticin (G418) - 600μg/ml - 12days
Reference:
S. Yamaguchi, Y. Kazuki, Y. Nakayama, E. Nanba, M. Oshimura and T. Ohbayashi 2011. A Method for Producing Transgenic Cells Using a Multi-Integrase System on a Human Artificial Chromosome Vector