NIH3T3

 
Species source: mouse
Organ source: embryo
Plasmid:
  • pCDNA3.1Neo
  • pcDNA3.1/zeo
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Geneticin (G418) - 600μg/ml - 14days
  • Zeocin - 800μg/ml - 14days
Reference:
  • P. Y. Ke, Y. Y. Kuo, C. M. Hu and Z. F. Chang 2005. Control of dTTP pool size by anaphase promoting complex/cyclosome is essential for the maintenance of genetic stability