GLUTag

 
Plasmid:
  • pcDNA3.1(+)
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Geneticin (G418) - 200μg/ml - 2days
Reference:
  • H. Bara and A. Sambanis 2008. Insulin-secreting L-cells for the treatment of insulin-dependent diabetes