2BS

 
Plasmid:
  • pcDNA SIRT1
  • pcDNA H363YSIRT1
  • pcDNA 3.1B
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Geneticin (G418) - 100μg/ml -
Reference:
  •  0.