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Antibiotics
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ES cell
ES cell
Plasmid:
Mrad9neo/loxP
Culture/Media:
Dulbecco's modified Eagle's medium
Temperature:
37
Atmosphere:
5% CO2
Antibiotics:
Geneticin (G418) - 200μg/ml -
Reference:
P. Roos-Mattjus, K. M. Hopkins, A. J. Oestreich, B. T. Vroman, K. L. Johnson, S. Naylor, H. B. Lieberman and L. M. Karnitz 2003. Phosphorylation of human Rad9 is required for genotoxin-activated checkpoint signaling