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Antibiotics
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HeLa-TO
HeLa-TO
Plasmid:
pTRE/TrxR2DN
pHyg
Culture/Media:
Dulbecco's modified Eagle's medium
Antibiotics:
Hygromycin - 100μg/ml - 2weeks
Geneticin (G418) - 100μg/ml - 2weeks
Reference:
M. R. Kim, H. S. Chang, B. H. Kim, S. Kim, S. H. Baek, J. Hye Kim, S. R. Lee and J. R. Kim 2003. Involvements of mitochondrial thioredoxin reductase (TrxR2) in cell proliferation