MEFs

 
Plasmid:
  • pQCXIP
  • pVSVG
Culture/Media: Dulbecco's modified Eagle's medium
Antibiotics:
  • Puromycin - 2μg/ml - 2weeks
Reference:
  • H. Huang, S. Y. Choi and M. A. Frohman 2010. A quantitative assay for mitochondrial fusion using Renilla luciferase complementation