U251

Cell-Lines
Plasmid/Vector
Antibiotics

Plasmid:	pMGPac pCI-rCD19-neo HyMP1-pEK
Culture/Media:	Dulbecco's modified Eagle's medium
Temperature:	37
Atmosphere:	5% CO2
Antibiotics:	Hygromycin B - 0.2μg/ml - Geneticin (G418) - 0.25μg/ml -
Reference:	L. J. N. Cooper, Z. Al-Kadhimi, L. M. Serrano, T. Pfeiffer, S. Olivares, A. Castro, W. C. Chang, S. Gonzalez, D. Smith and S. J. Forman 2005. Enhanced antilymphoma efficacy of CD19-redirected influenza MP1–specific CTLs by cotransfer of T cells modified to present influenza MP1

This project is supported byTOKU-Ewhich specializes in manufacturing ultra-pure antibiotics for a broad spectrum of biotechnology applications as well as for the pharmaceutical industry. TOKU-E is a global leader in biotechnology innovation, offering great benefits and applications to the biopharmaceutical and diagnostic industries as well as for biotechnology research communities.
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