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Plasmid/Vector
Antibiotics
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HeLa S3
HeLa S3
Species source:
human
Organ source:
cervix; adenocarcinoma
Plasmid:
pPuro
Culture/Media:
Ham’s F-12 nutrient mixture
Temperature:
37
Antibiotics:
Puromycin - 2μg/ml -
Reference:
B. Spänkuch, Y. Matthess, R. Knecht, B. Zimmer, M. Kaufmann and K. Strebhardt 2004. Cancer inhibition in nude mice after systemic application of U6 promoter–driven short hairpin RNAs against PLK1