MCF7

 
Species source: human
Organ source: pleural effusion (metastasis); adenocarcinoma (mammary gland primary)
Plasmid:
  • pSilencer 3.1-H1
Culture/Media: Dulbecco's modified Eagle's medium
Antibiotics:
  • Puromycin - 0.5μg/ml - 7days
Reference:
  • D. R. Wonsey and M. T. Follettie 2005. Loss of the forkhead transcription factor FoxM1 causes centrosome amplification and mitotic catastrophe