Rat-1/Wnt-1

 
Plasmid:
  • pcDNA3-flag-DN-Tcf-4
  • pBABE-puro
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Puromycin - 1.5μg/ml - 2weeks
Reference:
  •  0.