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C166

 
Species source: mouse (transgenic)
Organ source: yolk sac; endothelial cell differentiation model; stem cell feeder layer
Plasmid:
  • psi–VEGF-C
  • psi-control
Culture/Media: Minimal essential medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • G418 sulfate - 400μg/ml -
Reference:
  • Z. Chen, M. L. Varney, M. W. Backora, K. Cowan, J. C. Solheim, J. E. Talmadge and R. K. Singh 2005. Down-regulation of vascular endothelial cell growth factor-C expression using small interfering RNA vectors in mammary tumors inhibits tumor lymphangiogenesis and spontaneous metastasis and enhances survival
 
 
 
This project is supported byTOKU-Ewhich specializes in manufacturing ultra-pure antibiotics for a broad spectrum of biotechnology applications as well as for the pharmaceutical industry. TOKU-E is a global leader in biotechnology innovation, offering great benefits and applications to the biopharmaceutical and diagnostic industries as well as for biotechnology research communities.
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