NIH/3T3

 
Species source: mouse
Organ source: embryo
Plasmid:
  • pHZ-attB
  • pHZ-attP
Culture/Media: Dulbecco's modified Eagle's medium
Antibiotics:
  • Hygromycin B - 200μg/ml -
Reference:
  • B. Thyagarajan, E. C. Olivares, R. P. Hollis, D. S. Ginsburg and M. P. Calos 2001. Site-specific genomic integration in mammalian cells mediated by phage {phi} C31 integrase