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Plasmid/Vector
Antibiotics
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NIH/3T3
NIH/3T3
Species source:
mouse
Organ source:
embryo
Plasmid:
pCMV-Int
pCMVSPORTβGal
Culture/Media:
Dulbecco's modified Eagle's medium
Antibiotics:
Geneticin (G418) - 650μg/ml -
Reference:
B. Thyagarajan, E. C. Olivares, R. P. Hollis, D. S. Ginsburg and M. P. Calos 2001. Site-specific genomic integration in mammalian cells mediated by phage {phi} C31 integrase