MCF7

 
Species source: human
Organ source: pleural effusion (metastasis); adenocarcinoma (mammary gland primary)
Plasmid:
  • pcDNA1
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO 2
Antibiotics:
  • Geneticin (G418) - 500μg/ml - 7days
Reference:
  • J. Jin, T. D. Mullen, Q. Hou, J. Bielawski, A. Bielawska, X. Zhang, L. M. Obeid, Y. A. Hannun and Y. T. Hsu 2009. AMPK inhibitor Compound C stimulates ceramide production and promotes Bax redistribution and apoptosis in MCF7 breast carcinoma cells