AtT20

 
Plasmid:
  • pcDNA3
Culture/Media: Dulbecco's modified Eagle's medium
Antibiotics:
  • Geneticin (G418) - 400μg/ml - 2days
Reference:
  • N. Brakch, F. Allemandou, C. Cavadas, E. Grouzmann and H. R. Brunner 2002. Dibasic cleavage site is required for sorting to the regulated secretory pathway for both pro and neuropeptide Y