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Plasmid/Vector
Antibiotics
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BJ
BJ
Species source:
human
Organ source:
skin (foreskin)
Plasmid:
pRS-PITX1
MSCV-Ras
MSCV-Blast
Culture/Media:
Dulbecco's modified Eagle's medium
Antibiotics:
Blasticidin - 5μg/ml - 4days
Reference:
I. G. M. Kolfschoten, B. van Leeuwen, K. Berns, J. Mullenders, R. L. Beijersbergen, R. Bernards, P. M. Voorhoeve and R. Agami 2005. A genetic screen identifies PITX1 as a suppressor of RAS activity and tumorigenicity