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Plasmid/Vector
Antibiotics
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Caco-2
Caco-2
Species source:
human
Organ source:
colon; colorectal adenocarcinoma
Plasmid:
pGEMT
pCMV-β-galactosidase
Culture/Media:
Dulbecco's modified Eagle's medium
Antibiotics:
Geneticin (G418) - 1mg/ml - 48h
Reference:
F. Boudreau, Y. Zhu and P. G. Traber 2001. Sucrase-isomaltase gene transcription requires the hepatocyte nuclear factor-1 (HNF-1) regulatory element and is regulated by the ratio of HNF-1 to HNF-1